Coding

Part:BBa_K4011008:Design

Designed by: Zixiang Zhou   Group: iGEM21_LINKS_China   (2021-10-21)


CBM3-NT2RepCT-CBM3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 91
    Illegal AgeI site found at 301
    Illegal AgeI site found at 1609
    Illegal AgeI site found at 1819
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. All codons were optimized for E. coli based on E. coli codon bias

2. Flexible linker GSGGGS used between first CBM3 and NT2RepCT, flexible linker GGGGS used between NT2RepCT and second CBM3.

3. 6x histag (MGHHHHHHM) added to C-terminal to allow for Ni-NTA purification.


Source

CBM3 is derived from Ruminiclostridium thermocellum. NT2RepCT is an artificial spider silk fibroin (Visit BBa_K3264000 for more).

References