Coding
Part:BBa_K4011008:Design
Designed by: Zixiang Zhou Group: iGEM21_LINKS_China (2021-10-21)
CBM3-NT2RepCT-CBM3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 91
Illegal AgeI site found at 301
Illegal AgeI site found at 1609
Illegal AgeI site found at 1819 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. All codons were optimized for E. coli based on E. coli codon bias
2. Flexible linker GSGGGS used between first CBM3 and NT2RepCT, flexible linker GGGGS used between NT2RepCT and second CBM3.
3. 6x histag (MGHHHHHHM) added to C-terminal to allow for Ni-NTA purification.
Source
CBM3 is derived from Ruminiclostridium thermocellum. NT2RepCT is an artificial spider silk fibroin (Visit BBa_K3264000 for more).